Abstract
Cl − transport was studied in the glioma cell line LRM55. Our results indicate that LRM55 cells contain two major Cl − transporters, an anion exchanger and a K +/Cl − cotransporter, and that these are similar to the Cl − porters found in primary cultures of astrocytes. The exchanger was studied by measuring fluxes of Cl − (as 36Cl −). 36Cl − flux was trans-stimulated by Cl − or HCO 3 − and was inhibited by SITS or furosemide. The K +/Cl − cotransporter was studied by measuring fluxes of 36Cl − and K + (as 86Rb +). External K + stimulated 36Cl − influx, and external Cl − stimulated 86Rb + influx. Furosemide, but not SITS, inhibited the K +/Cl − cotransporter. As in primary cultures of astrocytes, the steady-state concentration of Cl − in LRM55 cells was higher than that predicted from passive equilibration according to the membrane potential. LRM55 cells appear to be a good in vitro model for glial Cl − transport.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
