Abstract
SummaryProtoplast fusion has been an effective tool with several applications for citrus cultivar improvement. The evaluation of fruit characteristics of certain previously produced citrus somatic hybrids with ‘Page’ tangelo or ‘Murcott’ tangor as parents, has shown that some allotetraploids produce high quality fruit with direct fresh-fruit cultivar potential. Based on this information, additional somatic hybrids combining these parents with other high quality scions were produced as follows: ‘Murcott’ tangor + ‘Dancy’ tangerine, ‘Murcott’ + LB8-8 (‘Clementine’ ‘Minneola’), ‘Page’ tangelo + ‘Murcott’, ‘Page’ + LB8-9 (‘Clementine’ ‘Minneola’), ‘Page’+ (‘Clementine’ ‘Satsuma’), ‘Page’ + Orie Lee hybrid (‘Clementine’ ‘Murcott’), and ‘Page’ + ‘Ortanique’ tangor, respectively. Their tetraploid level was confirmed by flow cytometry, and their hybridity was verified by random amplified polymorphic DNA (RAPD) or simple sequence repeat (SSR) analysis. Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analysis from mitochondria (mt) and chloroplast (cp) DNA, and cpSSR analysis, were also applied to analyse their cytoplasmic genomes. These new hybrids have potential for good quality fruit with reduced seed numbers, and can also serve as tetraploid breeding parents for subsequent triploid production.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: The Journal of Horticultural Science and Biotechnology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
