Abstract

Citrus is one of the most important and widely grown fruit crop with global production ranking firstly among all the fruit crops in the world. Sweet orange accounts for more than half of the Citrus production both in fresh fruit and processed juice. We have sequenced the draft genome of a double-haploid sweet orange (C. sinensis cv. Valencia), and constructed the Citrus sinensis annotation project (CAP) to store and visualize the sequenced genomic and transcriptome data. CAP provides GBrowse-based organization of sweet orange genomic data, which integrates ab initio gene prediction, EST, RNA-seq and RNA-paired end tag (RNA-PET) evidence-based gene annotation. Furthermore, we provide a user-friendly web interface to show the predicted protein-protein interactions (PPIs) and metabolic pathways in sweet orange. CAP provides comprehensive information beneficial to the researchers of sweet orange and other woody plants, which is freely available at http://citrus.hzau.edu.cn/.

Highlights

  • Citrus is one of the most important and widely grown fruit crop in the world, with global production and total acreage ranking firstly among all the fruit crops

  • To intuitively provide the sweet orange genome sequence and annotation, we constructed Citrus sinensis annotation project (CAP), which is a portal site for various types of sweet orange data

  • Users can search the system by gene locus, Gene Ontology (GO) [5], InterPro category [6] or functional information

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Summary

Introduction

Citrus is one of the most important and widely grown fruit crop in the world, with global production and total acreage ranking firstly among all the fruit crops. Sweet orange is responsible for about 60% of production for both fresh fruit and processed juice consumption [1]. Besides their economical and nutritional importance, Citrus fruits have unique botanical characteristics such as nucellar embryony [2]. We sequenced the draft genome of a double-haploid sweet orange The double-haploid genome was assembled to 4,811 scaffolds with N50 equal to 1.7 Mb. The total contig length (320.5 Mb) covers about 87% of the sweet orange genome, and scaffolds were aligned and oriented to the Citrus linkage map, about 80% of the assembled genome was anchored to nine pseudo-chromosomes [3]. To intuitively provide the sweet orange genome sequence and annotation, we constructed Citrus sinensis annotation project (CAP), which is a portal site for various types of sweet orange data

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