Abstract
Solid-state cultivation (SSC) is the growth of microorganisms on solid supports in the absence or near absence of free water. Sugarcane bagasse and vinasse are generated abundantly in Brazil as byproducts from the ethanol industry, and bagasse has been used as solid matrix for SSC to produce a variety of bioproducts. The present study aimed to evaluate the SSC of Aspergillus niger on sugarcane bagasse impregnated with vinasse, with airflow rate and column bed height as scale-up criteria. Airflow rate of 0.8 L min-1 caused a citric acid productivity of 3.77 mg (L.h)-1 and a yield of 0.73 g g-1 at 100 mm bed height. On the other hand, an airflow rate of 3 L min-1 resulted in a productivity of 8.46 mg (L.h)-1 with 2.26 g g-1 of citric acid at 200 mm, and an estimated specific oxygen consumption rate of 0.008 mg per mg of biomass per hour.
Highlights
Citric acid (2-hydroxy-propane-1, 2, 3-tricarboxylic acid), an intermediate of the tricarboxylic acid (TCA) is an organic acid of huge commercial importance, due to its applications as a preservative, sequestrant, flavorant, acidulant, emulsifier and buffering agent, being used in food, beverages, pharmaceutical, cosmetics and nutraceutical products (Verhoff, 2005; Yin et al, 2015; Wang et al, 2017)
The present study aimed to extend the findings of previous research by focusing on airflow rate and column bed height as scale-up criteria for the solid-state cultivation of Aspergillus niger on sugarcane bagasse impregnated with vinasse
The results indicate similar citric acid production behavior until day three on both axial positions, and the 100 mm curve tends to a maximum value while the 200 mm curve decelerates, suggesting limited oxygen conditions
Summary
Citric acid (2-hydroxy-propane-1, 2, 3-tricarboxylic acid), an intermediate of the tricarboxylic acid (TCA) is an organic acid of huge commercial importance, due to its applications as a preservative, sequestrant, flavorant, acidulant, emulsifier and buffering agent, being used in food, beverages, pharmaceutical, cosmetics and nutraceutical products (Verhoff, 2005; Yin et al, 2015; Wang et al, 2017). It is important to study oxygen transfer and bed heights because they are strongly related, since there could be a formation of oxygen gradients at some locations in the bioreactor, especially deeper into the bed, where the oxygen concentrations approach a value near zero (Muniswaran et al, 2002) In this context, the present study aimed to extend the findings of previous research by focusing on airflow rate and column bed height as scale-up criteria for the solid-state cultivation of Aspergillus niger on sugarcane bagasse impregnated with vinasse
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