Abstract
ABSTRACT This study aimed to evaluate the antioxidant potential of citric acid in plant protein based-diets offered to juvenile tambaqui, Colossoma macropomum. Four isonitrogenous and isoenergetic diets containing different levels of citric acid (0, 10, 20, and 30 g.kg−1) were formulated. Fish (n = 160; 27.56±1.03 g) were randomly distributed in 310-L water tanks (n = 16), with four replicates per treatment. After the experimental period (80 days), animals were anesthetized, and blood was collected for cell count. They were [...]
Highlights
Soybean meal corresponds to one of the best amino acid profiles compared with other plant sources (Hertrampf and Piedad-Pascual, 2000)
This study aimed to evaluate the antioxidant potential of citric acid in plant protein based-diets offered to juvenile tambaqui, Colossoma macropomum
No significant differences were observed for thrombocyte, total leukocyte, lymphocyte, and positive granular leukocytes (LG-PAS) counts and immature leukocytes (Kruskal-Wallis, X2 = 9, P = 0.51; Table 2) in animals fed the experimental diets
Summary
Soybean meal corresponds to one of the best amino acid profiles compared with other plant sources (Hertrampf and Piedad-Pascual, 2000). High proportions of soybean meal in fish diets can inhibit growth, increase production of reactive oxygen species (ROS), and, affect oxidative homeostasis of fish (Chen et al, 2018; Zhang et al, 2016). The imbalance between the generation and elimination of ROS induces oxidative stress, which damages important biomolecules (Nimse and Pal, 2015). Free radicals generated in fish tissues are effectively eliminated by the antioxidant system, which consists of enzymes and nonenzymatic agents (Dong et al, 2013). Changes in antioxidant and defense capacity can be considered important indicators of the nutritional value of plant protein sources
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