Citric acid can enhance the uptake and accumulation of organophosphate esters (OPEs) in Suaeda salsa rhizosphere: Potential for phytoremediation.

Abstract

Bioaccumulation of organophosphate esters (OPEs) by plants has been widely studied, but how root exudates influence their bioavailability to plants is poorly understood. Here, we examined whether root exudates could promote desorption of OPEs, thereby enhancing bioavailability and subsequent accumulation potential. Root exudate components exert great influences on the sorption/desorption isotherms of OPEs in soils, resulting in activating OPEs and enhanced bioavailability. Among root exudate components, citric acid was confirmed to play a crucial role in driving OPEs, with 77.7-90.3% attribution. Citric acid at rhizosphere levels (0.01-0.4mM) can successfully reduce OPEs sorption to soils by decreasing electrostatic interaction, ligand exchange, and hydrophobic force. Pot experiments indicated that the addition of citric acid can significantly increase OPEs dissolution and bioaccumulation from the rhizosphere soil to Suaeda salsa. A higher level of citric acid in rhizosphere soil resulted in a higher accumulation of OPEs in Suaeda salsa, which was partly attributed to the enhanced OPEs mobility, and the increased root lengths (13.4-29.0%) and tip numbers (60.2-120%), promoting OPEs uptake by roots. Our findings suggest the activation process of OPEs in soils by citric acid at rhizosphere levels and provide insights into designing LMWOAs-enhanced phytoremediation techniques in natural environment.