Abstract
AbstractThis study examined how Citrus herbal medicines—Citri Reticulatae Pericarpium (CRP), Citri Reticulatae Viride Pericarpium (CRVP), Aurantii Immaturus Fructus (AIF) and Aurantii Fructus (AF)—affect the differentiation of 3T3‐L1 adipocytes. Eight days after induction for differentiation with 3‐isobutyl‐1‐methylxanthine, dexamethasone and insulin (MDI) medium and simultaneously with the tested CRP, intracellular triacylglycerol accumulations of 3T3‐L1 cells were significantly (P < 0.05) reduced compared with those for CRVP, AIF and AF and those of the vehicle control. This suppression affect was dose‐dependent, and decreases in triacylglycerol production of 12.6, 18.7, 34.05 and 49.6% were observed for CRP at concentrations of 50, 100, 150 and 200 µg mL−1 respectively. Additionally, the expression of key transcription factors for the 3T3‐L1 adipogenesis gene, including PPAR‐γ, C/EBP‐α and SREBP‐1, was markedly reduced by CRP treatment. These results suggest that dietary CRP suppresses 3T3‐L1 differentiation by down‐regulation of adipogenic transcription factors. Experimental data may prove useful in further medical examination of the use of CRP for body weight control. Copyright © 2007 Society of Chemical Industry
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