Abstract
A sensitive assay for citrate was developed. Citrate was incubated with 50μM Eu3+-tetracycline and the complex separated using capillary electrophoresis utilizing post-column laser-induced luminescence detection in a sheath flow cuvette. Signal was linear with citrate concentration from 10μM to 200nM. Injection volumes were 320 pL. For the 200nM sample, this corresponds to the injection of 64 amoles of citrate. Separation time was < 90s with a total run time of 5min. As an application the method was used to analyze citrate in agricultural and medicinal products. The method was also used to develop an assay for the enzyme citrate synthase.
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