Citotoxicity of nonlatex elastomeric ligatures of orthodontic use

Rogério Lacerda-Santos,Paulyana Pryscilla De Melo Freire,Allana Roberta Bandeira Pereira,Alane Sonally Benício Do Nascimento,Maria Teresa Villela Romanos,Matheus Melo Pithon

doi:10.1590/s1517-707620150001.0004

Abstract

The aim of this study is to evaluate cytotoxicity between polyurethane and non-latex orthodontic elastomeric ligatures. Seven elastomeric ligatures of black-coloured from different manufactures (3 non-latex, 3 polyurethane and 1 latex) were divided into 7 groups of 10 elastics each: Group UK (nonlatex, 3M Unitek), Group TP (nonlatex, TP Orthodontics), Group AO (nonlatex, American Orthodontics), Group O (Polyurethane, OrthoSource), Group M (Polyurethane, Morelli), Group TD (Polyurethane, Tecnident) and Group TC (latex - Control, TP Orthodontics). The cytotoxicity essay was performed using cell cultures (L-929 line cells, mouse fibroblast) that were submitted to the cell viability test with neutral red (dye-uptake) at 1, 2, 3, 7 and 28 days. Analysis of variance (ANOVA) with 1-way analysis followed by the Tukey post hoc test were employed (p .05). There was a statistically differences (p .05) between the groups O, M and TD at in all experimental times. It can be concluded that the nonlatex elastomeric ligatures of Unitek, TP Orthodontics, and American Orthodontics showed higher cell viability compared to others ligatures.

Highlights

The latex because of its applicability in the health area has been the subject of many studies [1,2,3,4,5]
The objective of the present in vitro study was to test the cytotoxicity of polyurethane and nonlatex orthodontic elastomeric ligatures of different manufactures
The samples were divided into 7 groups of 10 elastics each: Group UK, Group TP, Group AO, Group O (Polyurethane, OrthoSource, North Hollywood, CA, USA), Group M (Polyurethane, Morelli, Sorocaba, São Paulo, Brazil), Group TD (Polyurethane, Tecnident, São Carlos, São Paulo, Brazil) and Group TC

Summary

Introduction

The latex because of its applicability in the health area has been the subject of many studies [1,2,3,4,5]. L929 mouse fibroblasts [8], have been shown to behave to primary human gingival fibroblasts and, are a suitable in-vitro model to the toxicity test [9,10,11,12] Taken in conjunction, these findings are aligned with the notion that the orthodontic elastics citotoxicity may influence their clinical performance [4,13] with regard to tissue biocompatibility. These findings are aligned with the notion that the orthodontic elastics citotoxicity may influence their clinical performance [4,13] with regard to tissue biocompatibility In this line, the objective of the present in vitro study was to test the cytotoxicity of polyurethane and nonlatex orthodontic elastomeric ligatures of different manufactures

Objectives

Methods

Results

Conclusion