Abstract
Cistanche deserticola Ma (cistanche) is a traditional herb with a wide range of therapeutic properties. However, no evidence of cistanche's effect on adipogenesis has been found. The effect of cistanche that promotes the adipogenesis of 3T3-L1 preadipocytes was proved by using MTT spectrophotometry, Nile Red staining, Oil Red O staining and transcriptome sequencing technology. The mRNA level of key transcription factors for adipogenesis such as PPAR, AP2 and LPL were examined by RT-PCR. The results showed that the intracellular lipid content in cistanche treated cells were notably increased when compared with the non-treated cells. Between the differentiation and cistanche treated groups, the expression of adipogenesis related genes such as grow hormone releasing hormone (Ghrp), BCL2/adenovirus E1B interacting protein 3 (Bnip3) and Gastric inhibitory polypeptide receptor (Gipr) were significantly increased. Our findings also verified that cistanche promoted adipogenesis, which was accompanied by up-regulated level of Bnip3 and PPAR. This study could uncover new signaling pathways involved in adipogenesis regulation.
Highlights
Adipocytes are a key component of adipose tissue, and their proliferation and differentiation are linked to the tissue’s function
Cistanche promotes the adipogenesis of 3T3-L1 preadipocytes
Our results showed that cistanche at 0.5μg/ml had no cytotoxic effect on 3T3-L1 preadipocytes after 24h and 48h treatments (P
Summary
Adipocytes are a key component of adipose tissue, and their proliferation and differentiation are linked to the tissue’s function. Adipocyte metabolism is abnormal in a range of disorders, including obesity, nutritional insufficiency and diabetes [1]. The study of adipocytes in vitro has become a popular issue in recent years due to the rising prevalence of lipid metabolism disorders. The 3T3-L1 preadipocytes are one of the most often used cells to create adipocytes models, and under the right conditions, they can be differentiated into adipocyte cells [2]. Knowing the key factors and signaling pathways in the process of adipogenesis could lead to a more effective strategy for treating lipid metabolism problems. It is vital to investigate the critical components and signaling pathways involved 3T3-L1 preadipocyte adipogenesis
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