Abstract
AbstractRNA has received enormous attention as a biomolecule in the fields of medicine, public health, and biotechnology. Over the last decade, various types of RNA molecules have been exploited as valuable drug targets by different classes of pharmaceutical agents in combating infections and diseases. In this work, we have investigated the binding of Cisplatin to RNA within double stranded RNA : DNA hybrid structures. UV melting and Isothermal Titration Calorimetry studies reveal that Cisplatin binding leads to destabilization of hybrid duplex likely due to Cisplatin‐RNA adduct formation. Gel electrophoretic analysis using an in vitro CRISPR‐Cas9 model system shows that Cisplatin binding to guide RNA leads to a concentration‐dependent reduction in target DNA cleavage.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
