Cisplatin and carboplatin can cause alteration of protein expression in human renal tubular epithelial cells

Abstract

Proteomic techniques are a powerful tool in nephrology research and allows the identification of important biomarkers. Using two‐dimensional polyacrilamide gel electrophoresis (2‐DE), we identified an alteration of protein expression in human renal tubular epithelial cells (HK‐2) induced by the treatment with anticancer drugs. HK‐2 were randomized in control (CT‐not treated), cisplatin (CPT‐100 µM) and carboplatin (CB‐50 µM). Sample protein (500 µg) was applied to a IPG strip (pH 3.0‐ 10.0), and electrophoresis ran under denaturing conditions. Gels were stained with coomassie G‐250, scanned and analysed by PdQuest software. Analysis showed a total of 124 spots in CT, 122 in CPT and 127 in CB. Using quantitative analysis (minimum difference of 5x expression between CT and treated groups) we identified a down‐regulation of 8 spots between CT and CPT groups, and also a down‐regulation of 12 spots of CPT and CB, compared with CT group. There was an up‐regulation of spots on CB and CPT groups (14 and 12 spots, respectively) compared with CT, and there was a correpondence on the up‐regulation of 11 spots, between both treatments and CT. Qualitative analysis (100x background) showed that some proteins are only expressed on CT (5 spots), CPT (12 spots) or CB (7 spots) groups. Our results, show that the treatment with anticancer drugs may induce alterations on protein expression in renal cells, which may be related to chemotherapy nephrotoxicity. The identification of these proteins may bring important information for new drug development.Financial support: Fapesp