Abstract
We read with great interest the article by Hoff et al1 on the applicability of visualization of fluorescence in situ hybridization (FISH) probes by dual-color chromogenic in situ hybridization (CISH) and totally agree about the need to perform studies on a larger number of samples to confirm their data. In our laboratory, we have been using this technique for 1 year, and to date our sample is composed of 40 cases tested for HER2 /centromere 17 status by FISH and subsequent CISH conversion. In our sample, the concordance between FISH and CISH assays is 100%, in complete agreement with the results reported by Hoff et al.1 As a matter of fact, as shown in Figure 1, all pairs of ratio values were consistently above or …
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