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Abstract
Twelve typical commercial hydrolases and crude cell extract of Bacillus subtilis ATCC6633 were tested for their activity to synthesize cis-7-phenylacetamido-3-propenyl-cephalosporanic acid (cis-GPRA) by enzymatic removal of a p-methoxybenzyl protecting group from p-methoxybenzyl-7-phenylacetylamino-3-propenyl-3-cephem-4-carboxylate (GPRE) in aqueous– organic solvents. No activity was detected for 12 commercial enzymes. However, the crude cell extract showed significant activity for removing the p-methoxybenzyl protecting group from GPRE to produce cis-GPRA. The yield of cis-GPRA reached 73% with the initial reaction rate of 10.2 μM h−1 under the optimized reaction conditions.
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