Abstract
Arachidonate stimulates the production of large quantities of superoxide by human neutrophils: 93.8 +/- 12.5 S.D. nmol of O2(-)/min/10(7) cells. This rate is comparable to that observed with the most effective neutrophil-stimulating agents previously reported. Other cis-unsaturated fatty acids are also capable of eliciting this response, the order of effectiveness being: arachidonate greater than gamma-linolenate greater than linoleate greater than oleate. Linolelaidate, myristate, and palmitate are ineffective. These data are discussed in relation to recent reports concerning the oxidation of arachidonic acid by human neutrophils and by a cell-free system that generates superoxide and hydrogen peroxide.
Highlights
Arachidonate stimulates the production of large, oleicacid (Sigma grade), 11-eicosaenoic acid
Myristate, and palmitate are ineffective. These data are Methods discussed in relation to recent reports concerning the oxidation of arachidonic acid by human neutrophils and by a cell-free system that generates superoxide and hydrogen peroxide
Preparation of Neutrophils-Human neutrophils werepurified from blood by the method of Skoog and Beck,with a previously described modification,except that hypotonic lysiswas performed twice to remove erythrocytes
Summary
From the Departmentof Biological Chemistry, Haruard Medical School, Boston, Massachusetts, 02115. Arachidonate stimulates the production of large (grade III), oleicacid (Sigma grade), 11-eicosaenoic acid This rate is comparable to that observed with the most effective neutrophil-stimulating agents previously reported. These data are Methods discussed in relation to recent reports concerning the oxidation of arachidonic acid by human neutrophils and by a cell-free system that generates superoxide and hydrogen peroxide. Preparation of Neutrophils-Human neutrophils werepurified from blood by the method of Skoog and Beck (lo),with a previously described modification (ll),except that hypotonic lysiswas performed twice to remove erythrocytes This procedure resulted in cell preparations that contained >90% neutrophils. Tion of their plasmalemma bay variety of surface active agents (for review, see Refs. 4-6).Of particular relevance is the fact
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