Cis-diammineplatinum(II) forms a macrochelate with 2′-deoxycytidine 5′-monophosphate (dCMP2–)! Reactivity and acid-base properties of cis-Pt(NH3)2(dCMP)

Abstract

The synthesis of cis-Pt(NH3)2(dCMP) is reported and by various physico-chemical methods it is demonstrated that it is a macrochelate in which Pt(II) is bound simultaneously to the N3 site of cytosine in dCMP2– and to a phosphate-oxygen atom. According to the NOESY spectra (cross-peaks between cytosine H6 and H2′ and H3′) the cytosine ring adopts an anti orientation. Highly unusual is the significant (1 ppm) downfield shift of the sugar proton H5″ in the 1H-NMR spectrum and the sensitivity of the cytosine H6 resonance on the protonation state of the phosphate group. Based on these three features a geometry for the macrochelate is proposed. The compound is a major product of the reaction of cis-[Pt(NH3)2(H2O)2]2+ with dCMP2– at neutral pH, but it even forms at pH 5. By applying pD-dependent NMR spectroscopy (1H, 31P) and potentiometric pH titration, it is demonstrated that the Pt-coordinated phosphate group can be protonated (pK a/1=3.21±0.10 and 3.31±0.05, respectively), and 1H- and 31P-NMR spectra also indicate deprotonation (pK a/2=13.35±0.25) of the exocyclic amino group of the cytosine moiety. The metal ion binding affinity of cis-Pt(NH3)2(dCMP) is very small, as shown for Cu2+ (log K<0.6). The cis-Pt(NH3)2(dCMP) complex reacts with nucleosides and nucleotides (L′) by losing its chelate structure and forming mixed ligand complexes, cis-Pt(NH3)2(dCMP)(L′); this means that the phosphate group is released from the coordination sphere of Pt(II), indicating that the Pt(II)-O(phosphate) bond is not very strong.