Cis-Acting regulatory elements in the GAP-43 mRNA 3′-untranslated region can function in trans to suppress endogenous GAP-43 gene expression

Abstract

The expression of the GAP-43 gene is controlled partly by changes in the stability of its mRNA, a process that is mediated by the interaction of specific sequences in the 3′-untranslated region (3′UTR) with neuronal-specific RNA-binding proteins. Limiting amounts of these trans-acting factors are available in the cell, thus we proposed that overexpression of the GAP-43 3′UTR could affect the levels of the endogenous mRNA via competitive binding to specific RNA-binding proteins. In this study, we show that chronic expression of GAP-43 3′UTR sequences in PC12 cells causes the depletion of the endogenous mRNA and consequent reduction of GAP-43 protein levels. The levels of the mRNAs for c- fos, the amyloid precursor protein (APP) and the microtubule associated protein tau, all three containing similar 3′UTR sequences, were not affected by the treatment. These results thus suggest that the effect of excess GAP-43 3′UTR is specific for its corresponding mRNA. We also used an HSV (herpes simplex virus)-1 vector and a mammalian expression vector with an inducible promoter to acutely express a 10 to 50 fold excess of 3′UTR sequences. Under these conditions, we found that transient expression of the GAP-43 3′UTR was effective in inhibiting both GAP-43 gene expression and neurite outgrowth in nerve growth factor (NGF)-treated PC12 cells and in primary neuronal cultures. These results underscore the role of 3′UTR sequences in the control of GAP-43 gene expression and suggest that overexpression of specific 3′UTR sequences could be used as a potential tool for probing the function of other post-transcriptionally-regulated proteins during neuronal differentiation.