Abstract
Pseudomonas putida strain G7 cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase (NahB) and Comamonas testosteroni strain B-356 cis-2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase (BphB) were found to be catalytically active towards cis-2,3-dihydro-2,3-dihydroxybiphenyl (specificity factors of 501 and 5850 s−1 mM−1 respectively), cis-1,2-dihydro-1,2-dihydroxynaphthalene (specificity factors of 204 and 193 s−1 mM−1 respectively) and 3,4-dihydro-3,4-dihydroxy-2,2′,5,5′-tetrachlorobiphenyl (specificity factors of 1.6 and 4.9 s−1 mM−1 respectively). A key finding in this work is the capacity of strain B-356 BphB as well as Burkholderia cepacia strain LB400 BphB to catalyze dehydrogenation of 3,4-dihydro-3,4-dihydroxy-2,2′,5,5′-tetrachlorobiphenyl which is the metabolite resulting from the catalytic meta-para hydroxylation of 2,2′,5,5′-tetrachlorobiphenyl by LB400 biphenyl dioxygenase.
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