CircZNF652 accelerates the proliferation and migration of primary lung carcinoma cells by downregulating miR-766

Xiaobing Hong,Yongqing Chen,Danzhen Zhang

doi:10.4314/tjpr.v20i11.3

Abstract

Purpose: To explore the biological functions and molecular mechanism of circZNF652 involvement in primary lung carcinoma.Methods: CircZNF652 levels in primary lung carcinoma cases and controls were determined using quantitative real-time polymerase chain reaction (qRT-PCR). Its prognostic value in primary lung carcinoma was examined by depicting it with Kaplan-Meier curves. The biological functions of circZNF652 in regulating proliferative and migratory capacities in A549 and SPC-A-1 cells were analyzed from the curves. Interaction between circZNF652 and its downstream gene, miR-766, wasassessed, and their co-regulation on primary lung carcinoma was determined by rescue experiments.Results: CircZNF652 was abnormally and significantly upregulated in primary lung carcinoma cases (p< 0.05), resulting in a poor prognosis. The knockdown effect of circZNF652 attenuated the proliferative and migratory capacities of A549 and SPC-A-1 cells, and downregulated epithelial-mesenchymal transition (EMT)-associated genes. CircZNF652 bound and negatively regulated miR-766, a keydownstream gene involved in circZNF652-induced aggravation of primary lung carcinoma.Conclusion: CircZNF652 serves as an oncogene, triggering the aggravation of primary lung carcinoma by negatively regulating miR-766. The results of this study may provide new insights into the treatment of lung carcinoma.

Highlights

Primary lung carcinoma is one of the leading cause of cancer death in the world, with the number of diagnosed cases growing rapidly. [1]
Wound healing assay and Transwell assay yielded the conclusion that the knockdown of circZNF652 suppressed migratory capacity in primary lung carcinoma (Figure 2 D and E)
Protein levels of N-cadherin and Vimentin were downregulated, whereas E-cadherin was upregulated in A549 and SPC-A-1 cells transfected with si-circZNF652 (Figure 2 F and G)

Summary

Introduction

Primary lung carcinoma is one of the leading cause of cancer death in the world, with the number of diagnosed cases growing rapidly. [1]. Primary lung carcinoma is one of the leading cause of cancer death in the world, with the number of diagnosed cases growing rapidly. Non-small cell lung cancer (NSCLC) is a major histological subtype of lung cancer, accounting for 80 % of primary lung carcinoma cases [2]. Chemotherapy and radiotherapy are the main therapeutic strategies for managing primary lung carcinoma [3]. To improve the clinical outcomes in NSCLC patients, novel molecular targeted therapy has been utilized [4]. Individualized therapy is conducted in order to prolong the survival chances of advanced NSCLC patients [5]. Sensitive biomarkers which support individualized therapy for primary lung carcinoma are limited. Circular RNAs (CircRNAs) are a type of unique noncoding RNAs and they have been highlighted

Methods

Results

Conclusion