Circulatory microrna in acute myocardial infarction: A candidate biomarker for forensic investigation

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs regulated a gene expression which associated to many diseases. As they contribute to diverse biological process and respond to various kinds of cellular stress, their utility as diagnostic biomarkers have recently been explored. miRNAs have been detected in various type of human tissues and were used as novel biomarkers in clinical investigation for example; ischemic stroke, acute myocardial infarction (AMI) and several forms of cancer cells. Recently, circulatory miRNA was proved that It could be a potential biomarker to determine a cause of death. In order to verify the ability of miRNA as a diagnosis tool, 3 candidate miRNAs (miR-133a, miR-208b and miR-499) associated to an early response of cardiac injury were selected. Twenty case-control cohorts using post-mortem blood samples were studied for miRNAs expression and post-mortem stability. The expression level of miR-499 was significantly increased in AMI groups compared to controls. The postmortem blood was in vitro tested during 12–18h which reveal a persistent of miRNAs in early decomposed environment.