Circulating tumor cells (CTCs) in association to breast cancer subtypes and inflammatory pattern

Abstract

acid (C22:6!3, DHA), play multifunctional roles in human health and diseases. The traditional source of these fatty acids is fish. Since fish stocks are in global decline, new sources of omega-3 LC-PUFA have to be found. The aim of the present study was to find potential alternative sources of these fatty acids in the territory of The Republic of Kazakhstan. Twelve strains of filamentous fungi isolated from soil and water were screened for lipids and LCPUFA: Penicillium sp., Penicillium restrictum, Penicillium aculeatum, Penicillium anatolicum, Penicillium bilaiae, Penicillium commune, Penicillium raistrikii, Penicillium crustosum, Fusarium sp. (2 strains), Aspergillus sp. and Mucor circinelloides. These fungi were grown in Sabouraud medium for 10 days. Lipid contents of these strains ranged from 1.5% to 7.4% of dry weight. No LC-PUFA were found in P. commune, P. crustosum, Aspergillus sp., M. circinelloides, whereas other strains contained EPA ranging from 1.0% to 3.5% of the total fatty acids, P. restrictum accumulated the largest amount. The cultural conditions that can increase the production of EPA in P. restrictum (e.g. the addition of glucose, glycerin and zinc) are currently under investigation.