Circulating nitric oxide metabolites during luteolysis and the effect of luteinizing hormone on circulating nitric oxide metabolites in heifers

Abstract

Temporal relationships among circulating concentrations of nitric oxide metabolites (NOM), progesterone (P4), and luteinizing hormone (LH) within the hours of a PGFM pulse were studied during luteolysis in heifers. The peak of a PGFM pulse was designated Hour 0. All of the following increases and decreases were significant. Within a spontaneous PGFM pulse (experiment 1; n = 7), concentrations of P4 and LH decreased between Hours −1 and 0 and increased between Hours 0 and 1; NOM increased between Hours −1 and 2. In experiment 2, PGFM pulses were simulated by intrauterine infusion of PGF2α (PGF group, n = 6), and another group was also treated with acyline to inhibit LH secretion (acyline-PGF group, n = 6). Averaged over the two groups, concentration of P4 decreased between Hours −2 and 0, increased (rebounded) between Hours 0 and 1, and decreased after Hour 2. In the PGF group, concentration of LH decreased between Hours −2 and −0.5 and increased between Hour 0 and Hour 1.5 to a maximum at Hour 1.5; NOM decreased between Hours −2 and −1.5 and increased between Hours 0 and 1.5. In the acyline-PGF group, the effect of hour was not significant for concentrations of LH and NOM. The absence of an increase in NOM concentration when LH was inhibited is a novel finding. The hypotheses were supported that concentrations of LH and NOM are temporally related, and LH has a role in the increase in NOM within the hours of a PGFM pulse.