Abstract
Background & Objective:The high-throughput analysis of circulating microRNAs (miRNAs) is an active area of biomarker research. The oral cancer remains a common cancer among Pakistani males that continues to present at an advance stage, thus exhibiting poor survival. MiRNA 21 (miR-21) is the most consistently over-expressed miRNA in different types of tumor tissues. However, information regarding expression of miR-21 in plasma remains to be resolved. Therefore, present study was designed to investigate if miR-21 was expressed in plasma of patients with oral cancer, and further explore its diagnostic and prognostic potential.Methods:Present study was conducted at Ziauddin University and Karachi Institute of Radiotherapy and Nuclear Medicine (KIRAN). Histologically confirmed cases of oral squamous cell carcinoma were recruited from Oncology Department of Ziauddin Hospital between 2013 and 2017. Controls were carefully selected after considering age, gender and socioeconomic condition. MiRNA was extracted and immediately reverse transcribed to cDNA. MiR-21 expression was evaluated using probes specifically designed for Real time quantitative polymerase chain reaction.Results:A significant over expression of miRNA 21 was observed in histologically confirmed cases as compared to controls. The increased expression of miRNA 21 was also reported to be associated with tumor size, metastasis and local invasion (p<0.05).Conclusion:The expression of circulating miR-21 in plasma samples of oral cancer patients makes it a promising diagnostic and prognostic marker.
Highlights
Oral cancer contributes to a significant proportion of cancer related morbidity and mortality in Pakistan.[1]
For cDNA preparation we added MMLV RT 4 μl, dNTPs 2 μl, DTT 0.5 μl, Oligo dT primer 1μl, reverted RT 1 μl, RNAse inhibitor 1 μl, distilled H2O 3.25 μl and eluted miRNA 4 μl to prepare a final volume of 17 μl. cDNA was prepared according to the set protocol as follows, incubation at 42o C for 50 min, 70oC for 10 min and 4oC
A higher expression of MiRNA 21 (miR-21) in patients with oral squamous cell carcinoma was observed. miR-21 is amongst the first miRNA observed to be expressed in serum
Summary
Oral cancer contributes to a significant proportion of cancer related morbidity and mortality in Pakistan.[1]. Most tragic part is that majority of the patients are diagnosed at an advance stage.[2] In this scenario the major focus of the research should be to identify markers which could predict prognosis and help in streamlining individualized treatment. MicroRNAs can serve this purpose as recent researches have revealed their potential capability of being used as diagnostic, prognostic and predictive tool in cancers. The oral cancer remains a common cancer among Pakistani males that continues to present at an advance stage, exhibiting poor survival. Present study was designed to investigate if miR-21 was expressed in plasma of patients with oral cancer, and further explore its diagnostic and prognostic potential. Results: A significant over expression of miRNA 21 was observed in histologically confirmed cases as compared to controls. Conclusion: The expression of circulating miR-21 in plasma samples of oral cancer patients makes it a promising diagnostic and prognostic marker
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