Abstract
Aim: The aim of the work was to study the circulating microRNA-133a levels in blood plasma of patients with arterial hypertension (AH), hypertensive heart disease (HHD), and left ventricular (LV) diastolic dysfunction (DD).Materials and Methods: A total of 48 patients with grade 2–3 AH and HHD at the age of 52.23 ± 7.26 (23 patients had LV DD [main group] and 25 patients had normal LV diastolic function [comparison group]) and 21 practically healthy individuals of comparable gender and age were examined. Diagnosis of AH and HHD was carried out according to the 2018 ESC/ESH recommendations. LV DD was determined according to the 2016 ASE/EACVI recommendations. Plasma microRNA-133a level was obtained by polymerase chain reaction using the CFX96 Touch System (BioRad), ≪TaqMan microRNA Assay≫ and ≪TaqMan® Universal PCR Master Mix≫ reagent kits (Thermo Fisher Scientific, USA).Results: We have found that in patients from the main and comparison groups plasma microRNA-133a levels were significantly lower than in practically healthy individuals (0.094 [0.067, 0.147]) and (0.182 [0.102, 0.301]) vs. (0.382 [0.198,0.474]), p = 0.002 and p = 0.04, respectively. In all this among patients with AH, HHD, and LV DD, plasma microRNA-133a levels were significantly lower than in patients with AH, HHD, and normal diastolic function (p = 0.03). In the main and comparison groups there was a statistically significant negative relationship between plasma microRNA-133a level and left ventricular mass index (LVMI) (R = −0.40, p = 0.003 and R = −0.35, p = 0.04, respectively).Conclusions: The findings suggest the significant role of decreased microRNA-133a levels in blood plasma of patients with AH in the pathogenesis and development of both HHD and LV DD.
Highlights
Arterial hypertension (AH) is a major public health problem due to its high prevalence all around the globe
Focusing on the hypertensive heart disease (HHD) it is important to note that chronically increased left ventricular (LV) workload in hypertensive patients can result in LV hypertrophy, impaired LV relaxation, development of LV diastolic dysfunction, left atrial enlargement, an increased risk of arrhythmias, especially atrial fibrillation, and an increased risk of heart failure with preserved ejection fraction and heart failure with reduced ejection fraction [1, 3, 4]
In the main and control groups, the NT-proBNP levels did not exceed 125 pg/mL, which indicated the absence of heart failure in the examined patients (Table 1)
Summary
Arterial hypertension (AH) is a major public health problem due to its high prevalence all around the globe. Elevated blood pressure (BP) may lead to the development of changes in major organs fed by the circulatory system, such as heart, kidneys, brain, and eyes. These changes are grouped under the term “target organ damage” or “hypertensionmediated organ damage.”. Focusing on the HHD it is important to note that chronically increased LV workload in hypertensive patients can result in LV hypertrophy, impaired LV relaxation, development of LV diastolic dysfunction, left atrial enlargement, an increased risk of arrhythmias, especially atrial fibrillation, and an increased risk of heart failure with preserved ejection fraction and heart failure with reduced ejection fraction [1, 3, 4]
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