Circulating lncRNA DANCR as a potential auxillary biomarker for the diagnosis and prognostic prediction of colorectal cancer

Xianjuan Shen,Xiaopeng Cui,Zhiwei Fan,Yajing Xue,Hui Cong,Xudong Wang,Shaoqing Ju

doi:10.1042/bsr20191481

Xianjuan Shen, Xiaopeng Cui + Show 5 more

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https://doi.org/10.1042/bsr20191481

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Journal: Bioscience reports	Publication Date: Mar 27, 2020
Citations: 31	License type: CC BY 4.0

Affiliation: Nantong University, Nantong Tumor Hospital

Abstract

Studies have shown that long non-coding RNAs (lncRNAs) play vital roles in the development of cancer, including colorectal cancer (CRC). Our purpose is to validate the diagnostic value of serum differentiation antagonizing non-protein coding RNA (DANCR) in CRC by focusing on its expression and clinical application. lncRNA expression profiles of CRC patients were obtained and analyzed by repurposing the publically available microarray data. Tissue or serum specimens were obtained from 40 patients with primary CRC, 10 patients with recurrent CRC, 40 patients with colorectal polyps, and 40 healthy controls. It was found that DANCR level in the CRC tissue and serum was significantly increased, and serum DANCR expression was decreased in post-operative patients as compared with that in pre-treatment patients and recurrent patients. In addition, serum DANCR expression was significantly correlated with different TNM stages. Correlation analysis of DANCR and other diagnostic indicators showed that the serum DANCR expression level was significantly correlated with CA199 but not with CEA in CRC patients. As for diagnostic efficiency by ROC analysis, the area under the curve (AUC) of serum DANCR was higher than that of CEA and CA199 in CRC group vs. colorectal polyp group. Simultaneous detection of DANCR, CEA and CA199 yielded the highest sensitivity and AUC as compared with either of them alone. Taken together, serum DANCR was up-regulated in CRC patients and high expression of DANCR may prove to be a potential biomarker for the diagnosis of CRC.

Highlights

Colorectal cancer (CRC) is a common human malignancy, ranking as the third leading cause of cancer-related deaths [1]
We selected an long non-coding RNA (lncRNA), named differentiation antagonizing non-protein coding RNA (DANCR), which was up-regulated in CRC
The data from starBase database that derived from the Cancer Genome Atlas (TCGA) database show that the expression of DANCR in CRC is higher than normal (Figure 1B)

Summary

Introduction

Colorectal cancer (CRC) is a common human malignancy, ranking as the third leading cause of cancer-related deaths [1]. Despite the major advances in diagnosis and treatment, CRC remains an intractable malignancy with a poor 5-year survival rate partly due to its susceptibility to metastasis, recurrence and drug resistance, as well as the lack of biomarkers for early diagnosis [2]. Colonoscopy is the gold standard for CRC diagnosis but its invasiveness has limited the wider use for large-scale screening. The existing diagnostic marker CEA has a limited utility for CRC because of its low sensitivity, especially for early diagnosis [3]. An ideal approach for early-stage detection should have high sensitivity and specificity

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