Circulating HDL and Non-HDL Associated Apolipoproteins and Breast Cancer Severity.

Christine Bobin-Dubigeon,Valentin Blanchard,Jean-Marie Bard,Mikaël Croyal,Hassan Nazih

doi:10.3390/jcm11051345

Abstract

Plasma lipids are carried within lipoproteins with various apolipoprotein content. This study evaluates the interest of measuring the apolipoproteins of circulating lipoproteins in breast cancer. Patients with early-stage breast cancer (n = 140) were included. Tumors differed by the expression of estrogen and progesterone receptor (HR− and HR+ for negative and positive expression) and the proliferation marker Ki-67 (≤20% or ≥30%). Apolipoprotein concentrations were determined in plasma, HDL and non-HDL fractions, and results are given in mg/dL, median (25th–75th). Patients did not differ in their plasma and lipoprotein lipid concentrations. HDL apoC-I and non-HDL apoC-II were reduced (1.34 (1.02–1.80) vs. 1.61 (1.32–2.04), p = 0.04; 0.31 (0.18–0.65) vs. 0.63 (0.39–1.02), p = 0.01; respectively), in RH-/high Ki-67 patients in comparison to RH-/low Ki-67 patients, while plasma apoD and HDL apoD were higher (3.24 (2.99–4.16) vs. 3.07 (2.39–3.51), p = 0.04; 2.74 (2.36–3.35) vs. 2.45 (2.01–2.99), p = 0.04; respectively). When RH+/high Ki-67 patients were compared with RH+/low Ki-67 patients, HDL apoC-I and HDL apoC-III were higher (1.56 (1.20–1.95) vs. 1.35 (1.10–1.62), p = 0.02; 2.80 (2.42–3.64) vs. 2.38 (1.69–2.96), p = 0.02; respectively). The distribution of exchangeable apolipoproteins, such as apoC-I, apoC-II, apoC-III, apoD, between lipoproteins is linked to the severity of breast cancer.

Highlights

Among the last years, several reports evidenced that various metabolic disturbances related to obesity may be associated with an increased risk of breast cancer [1,2]
We aimed to evaluate the interest of measuring the apolipoprotein content within circulating lipoproteins in breast cancer patients
We showed that patients with breast cancer of various severity display similar concentrations of plasma and lipoprotein lipids but different concentrations of some apolipoproteins carried by High-Density Lipoproteins (HDL) and non-HDL

Summary

Introduction

Several reports evidenced that various metabolic disturbances related to obesity may be associated with an increased risk of breast cancer [1,2]. Clinical studies looking at the association between circulating cholesterol carried by Low-Density Lipoproteins (LDL) or High-Density Lipoproteins (HDL) with breast cancer have raised conflicting results. Apolipoproteins play essential roles in maintaining the structural integrity and functional specificity of plasma lipoproteins They are directly involved in various metabolic processes of lipoproteins, including secretion, prevention of premature removal from the circulation, binding with cell-surface receptors and activation of lipolytic enzymes [5]. Besides their role in lipoprotein metabolism, apolipoproteins were shown to be involved in the development of breast cancer, as nicely reviewed recently [6]. The apoE plasma concentration was positively associated with breast cancer malignancy [10]

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