Circulating expression of Hsa_circRNA_102893 contributes to early gestational diabetes mellitus detection

Hongling Yang,Yan Long,Fangling Zeng,Ruihua Chen,Xiaoyan Zhang,Weitao Ye,Qiangsheng Gan,Chunyan Zhu,Jun Ma,Rehemayi Rehemutula

doi:10.1038/s41598-020-76013-5

Hongling Yang, Yan Long + Show 8 more

Open Access

https://doi.org/10.1038/s41598-020-76013-5

Copy DOI

Journal: Scientific Reports	Publication Date: Nov 4, 2020
Citations: 19	License type: open-access

Affiliation: Guangzhou Medical University

Abstract

Due to a poor availability of reliable biomarkers, detecting gestational diabetes mellitus (GDM) in early pregnancy remains a challenge. Novel biomarkers like Circular RNAs (circRNAs) may be a promising diagnostic tool. The aim of this study was (a) to identify circRNAs deregulated in GDM and (b) evaluate the potential of circRNAs in detecting GDM. The circRNAs expression profiling in 6 paired women (with and without GDM) was measured by microarray. The levels of five most relevant circRNAs were validated in 12 paired participants by qRT-PCR. To verify the reproducibility of qRT-PCR, significantly differential expressed circRNA levels were confirmed in 18 paired participants. A receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value. The areas under ROC curves of hsa_circRNA_102893 were 0.806 (95% CI 0.594–0.937) and 0.741 (0.568–0.872) in training set and test set, respectively. Circulating circRNAs reflect the presence of GDM. Hsa_circRNA_102893 may be a potential novel and stable noninvasive biomarker for detecting GDM in early pregnancy.

Highlights

Gestational diabetes mellitus (GDM) is a common metabolic complication of pregnancy, and is a leading cause of perinatal c omplications[1,2]
To identify if the candidate circRNAs validated by quantitative realtime reverse transcription-polymerase chain reaction (qPCR) could better discriminated gestational diabetes mellitus (GDM) from healthy pregnant women in different gestational weeks, we evaluated the performance of these circRNAs using the samples from the training set and test set, respectively
We report that hsa_circRNA_102893 may be a potential novel and stable biomarker for early GDM detection

Summary

Introduction

Gestational diabetes mellitus (GDM) is a common metabolic complication of pregnancy, and is a leading cause of perinatal c omplications[1,2]. To explore the molecular mechanism of GDM, many studies have focused on the differences in gene expression between pregnant women with GDM and pregnant women with normal glucose tolerance (NGT)[4,5,6]. The results of these studies indicate that changes in placental gene expression are involved in the occurrence of GDM. Circular RNAs (circRNAs) have emerged as important regulators of gene expression in some disease related pathologies. Little is known about circRNAs expression of GDM, which would show if there are new biomarkers serving as potential diagnosis and treatment targets for GDM.

Objectives

Methods

Results

Conclusion