Abstract
Absolute numbers of circulating basophils were measured in 55 atopic and 35 non-atopic individuals, using a flow cytometer for automated cell counting. They were significantly elevated in the atopic group (p less than 0.001), but the total leucocyte count was not significantly different between the two groups. Eosinophil counts, which correlated with the number of basophils for both populations, were also significantly raised in the atopic group (p less than 0.001). Individual variation in absolute basophil counts was not detected in sequential samples taken at daily intervals over 5 days, in 5 atopics and 4 non-atopics. In addition, no significant variation was detected in 6 atopics and 5 non-atopics over a period of up to 20 months.
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