Abstract
OBJECTIVES:Malignant melanoma (MM) is an invasive tumor that poses a threat to patient health. Circular RNAs (circRNAs) are important regulators of MM carcinogenesis. In this study, we investigated the expression characteristics and biological functions of, and mechanism underlying, circ_0119872 expression in MM.METHODS:Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was employed to examine the circ_0119872, microRNA (miR)-582-3p, and E2F transcription factor 3 (E2F3) mRNA expression levels in MM tissues and cell lines. Western blotting was performed to quantify E2F3 protein expression. MM cells with circ_0119872 knockdown were established, and cell counting kit 8 (CCK-8) and transwell assays were utilized to examine the function of circ_0119872 and its effects on the malignant characteristics of MM cells. The MiRDB and TargetScan databases were used to predict the target genes of miR-582-3p. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was used to explore the biological functions of the target genes of miR-582-3p. Additionally, a dual-luciferase reporter gene experiment was performed to verify the targeting relationship between circ_0119872 and miR-582-3p as well as that between miR-582-3p and E2F3.RESULTS:Circ_0119872 was remarkably upregulated in MM tissues and cell lines. Circ_0119872 knockdown suppressed the cell proliferation and metastasis In addition, miR-582-3p was identified as a downstream target of circ_0119872. The target genes of miR-193a-3p are involved in melanogenesis and cancer-related signaling pathways. Mechanistically, circ_0119872 facilitated MM progression by adsorbing miR-582-3p and upregulating E2F3 expression.CONCLUSION:Circ_0119872 is an oncogenic circRNA that participates in the promotion of MM progression by regulating the miR-582-3p/E2F3 axis.
Highlights
Malignant melanoma (MM) is a malignancy that originates in melanocytes [1]
Circ_0084043, whose expression is remarkably upregulated in MM tissues, enhances MM cell proliferation, migration, and invasion by regulating the miR-153-3p/Snail axis [4]; circ_0016418, whose expression is remarkably upregulated in MM tissues and cell lines, facilitates MM cell proliferation, migration, and epithelial-mesenchymal transition (EMT) by suppressing miR-625 expression and upregulating YY1 expression [5]; and circ_0025039 facilitates MM cell proliferation, invasion, and glucose metabolism by modulating the miR-198/CDK4
MiR-582-3p expression is remarkably downregulated in bone metastatic prostate cancer tissues, and miR-582-3p under-expression is linked to poor prognosis of the patients; miR-582-3p targets the TGF-b signaling pathway to impede cell migration, invasion, and bone metastasis of cancer cells [11]
Summary
Malignant melanoma (MM) is a malignancy that originates in melanocytes [1]. There are more than 200,000 new cases of MM worldwide each year, and its morbidity is increasing annually [2,3]. Identifying the mechanisms of MM development is essential for the development of novel drugs to treat this aggressive disease. Circular RNAs (circRNAs) are non-coding RNAs with covalently bonded closed-loop structures [4,5,6]. Over the past few years, with the development of high-throughput sequencing technology and bioinformatics, circRNAs have.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
