Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention.

Xinyao Tian,Zhe Yang,Lin Zhou,Yuanxing Liu,Yan Hu,Shusen Zheng,Haiyang Xie

doi:10.3389/fmed.2021.626948

Abstract

Ischemic preconditioning (IPC) represents an effective intervention to relieve hepatic ischemia-reperfusion injury (IRI). Systematic detection of circRNA expression revealing the protection effect of IPC still remains to be elucidated. Here, we applied a microarray to detect circRNA and mRNA expression in ischemic liver with and without IPC (n = 3 in each group). Compared with the sham group, there were 39 circRNAs and 432 mRNAs increased and 38 circRNAs and 254 mRNAs decreased (fold change ≥1.5, P < 0.05) in the group of hepatic IRI. As the result of IPC intervention, 43 circRNAs and 64 mRNAs were increased, and 7 circRNAs and 31 mRNAs were decreased in the IPC group when compared with IRI. We then identified circRNA_017753 as the most possible target that may closely relate to IPC protective signaling and predicted Jade1 as the target related to circRNA_017753. Three possible circRNA–miRNA–mRNA axes were constructed that may play a vital role in protective mechanisms in IPC. The study for the first time systematically detects the dysregulated circRNAs and mRNAs in response to hepatic IRI and IPC intervention. Our profile and bioinformatic analysis provide numerous novel clues to understanding the pathophysiologic mechanism of IPC protection against hepatic IRI.

Highlights

Hepatic ischemia-reperfusion injury (IRI) occurs in clinical circumstances, including hepatic resection, transplantation, liver trauma, or septic shock [1]
The blood index of hepatic injury after hepatic IRI with or without Ischemic preconditioning (IPC) were tested to identify the beneficial effect of IPC in the study
Serum ALT and AST were upregulated after hepatic IRI, and IPC remarkably decreased serum transaminase levels (Figure 1B)

Summary

Introduction

Hepatic ischemia-reperfusion injury (IRI) occurs in clinical circumstances, including hepatic resection, transplantation, liver trauma, or septic shock [1]. The reperfusion aggravates hepatic injury after ischemia. Concerning mechanisms involve microcirculatory failure, inflammatory cytokine release, and reactive oxygen species accumulation [2, 3]. In the surgical procedures of hepatic resections and liver transplantation, IRI contributes to organ damage, and reduces the long-term survival rates. Strategies to reduce hepatic IRI and improve patient outcomes is clinically important at any point.

Methods

Results

Conclusion