Abstract
Circular RNAs are a class of endogenous noncoding RNAs that play an important role in gene regulation. These RNAs are involved in the development and progression of various cancers, but their roles in gastric cancer have not yet been thoroughly elucidated. This study showed that hsa_circ_0000467 expression was higher in gastric cancer tissues than in corresponding adjacent tissues (P < 0.050) and that hsa_circ_0000467 expression levels were correlated with gastric cancer histological grade (P < 0.050). In addition, hsa_circ_0000467 was remarkably upregulated in gastric cancer cell lines (P < 0.001). Cell function experiments indicated that hsa_circ_0000467 downregulation decreased the proliferation and invasion ability of BGC-823 and SGC-7901 cells and the number of cells entering the G2/M phase. A direct binding interaction was detected between hsa_circ_0000467 and miR-326-3p by dual-luciferase reporter assays. In addition, the results showed that inhibition of miR-326-3p reversed the decreases in the proliferation and invasion of BGC-823 and SGC-7901 cells caused by hsa_circ_0000647 downregulation. Inhibition of miR-326-3p also decreased the number of cells entering the G2/M phase and the expression of cyclin D1. In conclusion, hsa_circ_0000467 plays a regulatory role in the development and progression of gastric cancer by regulating miR-326-3p, and this circRNA may be a potential diagnostic marker and therapeutic target of gastric cancer.
Highlights
Gastric cancer (GC) is a common digestive system tumor that occurs worldwide and is the second most common cause of cancer morbidity and mortality in China [1]
We evaluated the clinical significance of our findings. e effects of hsa_circ_0000467 downregulation on the proliferation, invasion, and cell cycle of GC cells were verified by CCK8 assays, Transwell assays, and flow cytometry
We selected hsa_circ_0000467 as our research focus, and the other circRNAs were studied by other members of our research team
Summary
Gastric cancer (GC) is a common digestive system tumor that occurs worldwide and is the second most common cause of cancer morbidity and mortality in China [1]. In 2015, approximately 498,000 people died of GC in China [2]. E 5-year survival rate of patients with early GC is more than 90%. Most patients have lost the opportunity for effective surgery when they are diagnosed, meaning that their prognosis is poor. Circular RNAs (circRNAs) exist widely in humans. Ese RNAs are covalently closed-loop structures that do not have 5′ to 3′ polarity or a polyadenylation tail [3]. CircRNAs are stable in nature and not cleaved by Ribonuclease R [4]. CircRNAs have been demonstrated to be critically involved in malignancies, such as liver cancer [7], bladder cancer [8], esophageal cancer [9], breast cancer [10], and prostate cancer [11]
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