Circular RNA hsa_circ_0000285 acts as an oncogene in laryngocarcinoma by inducing Wnt/β-catenin signaling pathway.

Abstract

Laryngocarcinoma is one of the most ordinary head and neck cancers worldwide. Recent studies have revealed that circular RNAs (circRNAs) act as an important role in malignant tumors and participate in tumorigenesis. The purpose of our work is to uncover how hsa_circ_0000285 functions in laryngocarcinoma. In this research, the Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was performed to monitor hsa_circ_0000285 expression in laryngocarcinoma samples. Besides, function assays were performed in laryngocarcinoma cells transfected with hsa_circ_0000285 shRNA or lentivirus. Furthermore, the RT-qPCR and Western blot assay were conducted to explore the target-signaling pathway of hsa_circ_0000285. Hsa_circ_0000285 expression was found to be upregulated in laryngocarcinoma samples compared with adjacent tissues. The function assays showed that the inhibition of the cell proliferation was induced via knockdown of hsa_circ_0000285 in laryngocarcinoma in vitro, while the promotion of cell apoptosis was induced via the knockdown of hsa_circ_0000285 in laryngocarcinoma in vitro. On the other hand, the overexpression of hsa_circ_0000285 had the opposite function. In addition, the Wnt/β-catenin signaling pathway was repressed via knockdown of hsa_circ_0000285 in laryngocarcinoma, while the Wnt/β-catenin signaling pathway was promoted via overexpression of hsa_circ_0000285 in laryngocarcinoma. In our study, hsa_circ_0000285 was first identified as a novel oncogene and could induce the Wnt/β-catenin signaling pathway in laryngocarcinoma.