Abstract
Circular RNAs (circRNAs) are a type of non-coding RNAs that have been identified as critical regulators in various diseases, especially in cancers. However, the expression profiles and functions of circRNAs in cervical cancer are still unclear. In present study, human circRNAs microarray were performed to screen the circRNAs expression in cervical cancer tissue. Microarray analysis revealed 45 significantly expressed circRNAs with 4 fold change. Among these up-regulated circRNAs, hsa_circ_0018289 was validated to be significantly up-regulated in 35 pairs of cervical cancer tissue compared with adjacent normal tissue and cell lines. Loss-of-function experiments revealed that, in vitro and in vivo, hsa_circ_0018289 knockdown inhibited the proliferation, migration and invasion of cervical cancer cells. Via bioinformatics prediction program and luciferase reporter assays, hsa_circ_0018289 was observed to directly bind to miR-497. Taken together, the results indicate that hsa_circ_0018289 plays important role in cervical cancer proliferation, migration and invasion, suggesting the miRNA ‘sponge’ of hsa_circ_0018289 and its oncogenic role on cervical cancer tumorigenesis.
Highlights
Cervical cancer is one of the most common gynecologic tumors and accounts for large percentage of tumor associated death worldwide [1, 2]
In initial stage of experiments, 4 pairs of cervical cancer tissue and adjacent noncancerous tissue were performed for circRNA expression profile
Heat map showed 45 significantly expressed circRNAs with 4 fold change (P
Summary
Cervical cancer is one of the most common gynecologic tumors and accounts for large percentage of tumor associated death worldwide [1, 2]. It must be admitted that multifarious molecular and pathways are involved in the carcinogenesis of cervical cancer, such as regulating proteins and non-coding RNAs (ncRNAs). The underlying functional molecular and regulation mechanisms in cervical cancer tumorigenesis are still unclear and need to be fully identified [5]. CircRNAs could much easier to be accumulated in cytoplasm or interstitial fluid, presenting higher concentration than corresponding linear RNAs. Increasing evidences have indicated that circRNAs take part in the regulation of tumorigenesis, including genesis, differentiation, migration and metastasis [10]. Due to the stable expression and abundance, circRNAs could act as valuable diagnostic marker for cancer detection. Hsa_circ_0005075, a new identified circRNA, is involved in the progression of hepatocellular carcinoma and could act as a diagnostic marker [12]
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