Abstract
Circular RNA (circRNA) is a type of non-coding RNA molecule that affects the cellular regulatory network by sequestering microRNA (miRNA) like a sponge. This study was performed to identify differentially-expressed circRNA in oral squamous cell carcinomas (OSCCs). By high-throughput sequencing, microarray circRNA expression profiles were acquired from patients with OSCCs (n = 8) and controls (n = 8), which totaled 1921 existing circRNA molecules and 10021 novel circRNA molecules. Most of the circular RNA is from exons and distributed in the No. 1 and 2 chromosomes. Eight up-regulated and down-regulated circRNA molecules were identified as differentially-expressed in OSCCs. Among this, the expression of circ_000334, circ_006740, and circ_006371 are significantly down-regulated in 42 pairs of samples, which means that these circRNA molecules might be implicated in oncogenesis and development of OSCCs.
Highlights
Head and neck squamous cell carcinoma (HNSCC), which affects 600,000 new patients each year, is the sixth most common malignancy worldwide[1, 2]
We found a large amount of circRNA in the eight pairs of oral squamous cell carcinomas (OSCCs) and adjacent normal tissue through high-throughput sequencing
A total of 11942 circRNA targets, including 1921 existing circRNA molecules and 10021 novel circRNA molecules, were detected in eight pairs of samples, the scatter plot of circRNA expression profile was used to assess the variations between the two groups (Figure 2)
Summary
Head and neck squamous cell carcinoma (HNSCC), which affects 600,000 new patients each year, is the sixth most common malignancy worldwide[1, 2]. It occurs in the oral cavity, oropharynx, larynx, and hypopharynx and accounts for over 90% of head and neck cancers in China [3]. The current preferred therapy for HNSCC usually requires multimodal therapy combining surgery, radiotherapy, chemotherapy, and biotherapy. Surgery and radiotherapy are the major treatments. Recent studies have shown that preoperative chemotherapy does not improve overall survival and preoperative radiotherapy increases the risk of complications [4]. One important reason is that the mechanism of squamous cell carcinoma is unclear
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