Circular RNA circYPEL2: A Novel Biomarker in Cervical Cancer

Xinyang Zhang,Chunjiang He,Haidong Ye,Rongyu Zhang,Xin Dong,Xiangfei Mei,Wenbo Chen,Yu Fang,Huan Liu,Siqi Yang

doi:10.3390/genes13010038

Xinyang Zhang, Chunjiang He + Show 8 more

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https://doi.org/10.3390/genes13010038

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Abstract

Cervical cancer (CC) is one of the most threatening diseases in women. Circular RNAs (circRNAs) have been reported to be cancer hallmarks, but typical circRNAs in CC were rarely indicated. Through high-throughput sequencing in CC and normal cervix tissues, circYPEL2 (hsa_circ_0005600) was proposed as a candidate circRNA. CircYPEL2 exhibited significantly high expression in CC tissue and strong stability in CC cell lines. Furthermore, knockdown and overexpression of circYPEL2 indicated the potential involvement in CC proliferation, migration and invasion. Finally, the downstream regulatory genes of circYPEL2 were investigated by knockdown experiment in CC cell lines with high-throughput sequencing. In summary, our work identified circYPEL2 as a potential biomarker for clinical research of cervical cancer.

Highlights

Cervical cancer (CC) is the fourth most common cancer in women, causing death in up to 55% of patients
Our work indicated that circYPEL2 may act as a potential biomarker in the development of CC
Overlap analysis revealed 59,093 circRNAs are only expressed in CC and 22,188 circRNAs are only expressed in normal samples (Figure 1A)

Summary

Introduction

Cervical cancer (CC) is the fourth most common cancer in women, causing death in up to 55% of patients. More than 100 types of HPV have been identified as high-risk viruses based on their oncogenic characteristics, among which HPV 16 and HPV 18 are the most critical carcinogenic subtypes [2]. With the widespread application of vaccines against HPV and early cancer screening, an increasing number of patients can be treated at the early stages of the disease [3]. The current method for CC screening is dependent on microscopic examination of cervical cells and HPV testing, which includes several complicated steps and is costly [4]. Identifying novel biomarkers for CC screening is a candidate procedure to improve the accuracy of diagnosis

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