Abstract
Circular RNAs (circRNAs), expressed abundantly and universally in various eukaryotes, are involved in growth and development of animals. Our previous study on circRNA sequencing revealed that circSVIL, an exonic circular, expressed differentially among skeletal muscle at 11 embryo age (E11), 16 embryo age (E16), and 1 day post-hatch (P1). In this study, we aim to investigate the effect of circSVIL on the development of skeletal muscle. We detected the expression level of circSVIL in embryonic leg muscle during E10 to P1. As a result, we found that circSVIL had a high expression level during late embryonic development of skeletal muscle. Through dual-luciferase assay, RNA immunoprecipitation and biotin-coupled miRNA pull down, we found chicken circSVIL could functions as miR-203 sponges and upregulated the mRNA level of c-JUN and MEF2C. In chicken, circSVIL could promote the proliferation and differentiation of myoblast, and antagonize the functions of miR-203. Altogether our data suggest that circSVIL promotes the embryonic skeletal muscle development by sequestering miR-203 in chicken.
Highlights
Skeletal muscle is the most important component of body, and it is directly correlated with meat production in domestic animals (Güller and Russell, 2010)
MiR-203 negatively regulates the proliferation of smooth muscle cells, and was detected abundant circSVIL Promotes Myoblast Proliferation and Differentiation expressed in C2C12 myoblasts, quail myoblasts and chicken skeletal muscle (Chen et al, 2006; Li et al, 2011; Liao et al, 2015)
The results showed that the expression of circSVIL increased sharply during E11–E14, and maintained at a high expression level from E14 to P1 (Figure 1C)
Summary
Skeletal muscle is the most important component of body, and it is directly correlated with meat production in domestic animals (Güller and Russell, 2010). MicroRNAs (miRNAs) have been extensively studied and characterized as crucial components within the regulatory network for muscle development. Several well-known myogenic miRNAs, such as miR1, miR-206, and miR-133, were reported to regulate muscle development by inhibiting target gene expression (Anderson et al, 2006; Chen et al, 2006). MiR-203 negatively regulates the proliferation of smooth muscle cells, and was detected abundant circSVIL Promotes Myoblast Proliferation and Differentiation expressed in C2C12 myoblasts, quail myoblasts and chicken skeletal muscle (Chen et al, 2006; Li et al, 2011; Liao et al, 2015). MiR-203 regulates fast muscle differentiation by targeting dmrt2a (Lu et al, 2017)
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