Circular RNA CircPGC expression level is decreased in gastric cancer tissues and it promotes migration of gastric cancer cells

Abstract

Objective To investigate the expression of CircPGC in gastric cancer and regulation on the migration of gastric cancer cell AGS. Methods Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to verify the level of CircPGC. The CircPGC overexpression vector, pcDNA3.1 (+ )-CircPGC, was constructed by seamless cloning method. The effect of CircPGC in migration of AGS cell was detected by scratch assay. Using GraphPad Prism 6.0 statistical software analysis, the measurement data were expressed as mean±standard deviation (Mean±SD), and t-test was used for comparison between groups. Results The expression level of CircPGC in human gastric cancer tissues (ΔCt=12.480±2.385) was significantly lower than that in normal gastric tissues (ΔCt=7.327±2.696) and adjacent tissues (ΔCt=7.122±3.030). Compared with the control group, the expression of CircPGC in AGS cell was significantly increased after transfection of pcDNA3.1(+ )-CircPGC plasmid (t=11.860, P<0.01). Overexpression of CircPGC promotes cell migration: The scratch test showed that the migration distance of AGS cells in the overexpressing group was (44.900±5.284)%, which was longer than the control group (10.010±1.597)% (t=10.580, P<0.01). Conclusion CircPGC is a circular RNA associated with migration of gastric cancer. Key words: Circular RNA circPGC; Gastric cancer; Migration