Abstract
Emerging evidence indicates that circRNAs play essential roles in tumorigenesis and development. However, the role of circRNAs in cervical cancer (CC) remains unclear. CircRNA microarrays performed on the immortal cervical cell line H8 and the cervical cancer cell line SiHa were used to identify a circRNA, termed circNRIP1 (hsa_circ_0004771), which was upregulated in SiHa. QRT-PCR confirmed that circNRIP1 was upregulated in CC tissues, where its expression was correlated with lymphovascular space invasion. Besides, both in vitro and in vivo experiments demonstrated that circNRIP1 promotes cell proliferation, migration, and invasion. Additionally, we found that miR-629-3p induced tumor suppression by regulating PTP4A1 and the ERK1/2 pathway. Finally, we confirmed that circNRIP1 exerts its effect, at least partially, by sponging miR-629-3p and thereby regulating the PTP4A1/ERK1/2 pathway. Therefore, circNRIP1 may be useful as a potential prognostic biomarker and therapeutic target in CC patients.
Highlights
Introduction According to the National CentralCancer Registry ofChina (NCCRC), cervical cancer is the sixth most common cancer in women in China, with ~111,000 newly diagnosed cases and nearly 34,000 new deaths in 20151
CircNRIP1 is upregulated in cervical cancer We performed a microarray analysis of the cervical cancer cell line, SiHa, and the immortalized cervical epithelial cell line, H8 (Fig. 1a)
The current study performed a microarray analysis on SiHa and H8 cell lines and identified a novel Circular RNA (circRNA), termed circNRIP1, which was significantly upregulated in the SiHa cell
Summary
Introduction According to the National CentralCancer Registry ofChina (NCCRC), cervical cancer is the sixth most common cancer in women in China, with ~111,000 newly diagnosed cases and nearly 34,000 new deaths in 20151. According to the Surveillance, Epidemiology, and End Results (SEER) registry, between 2009 and 2015, 36% and 15% of women, respectively, were diagnosed at regional and distant stages of cancer progression, despite the availability of viable prevention strategies and vaccines[2]. These women were considered incurable, as they had already lost the opportunity to undergo surgical resection of their lesions due to metastasis[3]. Some circRNAs, such as circ_0067934 and circRNA-000284, have been reported to function in the sponging of miRNAs8,12
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