Abstract
Background: CircRNAs have been found to play a crucial role in the pathological process of various kinds of diseases. However, the role of circRNAs in the formation and rupture of intracranial aneurysm is still unknown.Methods: Differentially expressed circRNAs profiles between superficial temporal arteries (n = 5) and intracranial aneurysms (n = 5) were analyzed using the Arraystar human circRNAs microarray. Quantitative real-time PCR was utilized to validate the differential expression of circDUS2. Fluorescence in situ hybridization (FISH) was meant for the location of circDUS2 in human brain vascular smooth muscle cell (HBVSMC). Structural analysis was used to speculate on the function of circDUS2.Results: Five hundred forty-three upregulated and 397 downregulated significantly in intracranial aneurysm as compared to superficial temporal arteries. Quantitative real-time PCR verified the elevated expression of the upregulated circDUS2. The FISH test revealed that circDUS2 is located in the cytoplasm of brain vascular smooth muscle cells.Conclusion: This study showed differential expression data of circRNAs between superficial temporal artery and intracranial aneurysm and revealed that circDUS2 is a potential molecular marker for intracranial aneurysm.
Highlights
Intracranial aneurysm (IA) is abnormal bulging brought about by structural damage to intracranial artery walls because of multiple factors
We detected a total of 13,174 circRNAs using the Arraystar human circRNA Microarray (Supplementary Table 4)
942 circRNAs dysregulated between STA and IA tissues, and 750 of them have been identified in circBase by other studies
Summary
Intracranial aneurysm (IA) is abnormal bulging brought about by structural damage to intracranial artery walls because of multiple factors. It is becoming increasingly clear that circRNAs play a crucial role in the pathological process of many kinds of cancers, such as colorectal cancer (Zeng et al, 2018), breast cancer (Pamudurti et al, 2017), and hepatocellular carcinoma (Yang et al, 2017). Research about the role of circRNAs in the formation and rupture of intracranial aneurysm is rare, and the overall pathophysiological contributions of circRNAs to intracranial aneurysm remain largely unknown. We performed bioinformatical analysis to explore the potential functions of circRNA in IA These data would lay a foundation for future investigations on the molecular functions of circRNAs in IA. The role of circRNAs in the formation and rupture of intracranial aneurysm is still unknown
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