Abstract
In recent years, the mechanism of cancer research has become hotspots of life science and medicine, especially due to the rapid development of molecular medicine and bioinformatics research. Similarly, the molecular mechanism also has received increasing attention in osteosarcoma (OS) research. Also, a considerable amount of research confirmed that circular RNAs (circRNAs) could regulate cancer cell growth and metastasis. This study aimed to explore the effect of a circRNA, circCCDC66, on OS and reveal its potential molecular mechanism. High circCCDC66 expression level was found in OS patient-derived tissue samples and OS cell lines by qRT-PCR. The abilities cell proliferation and metastatic of U2OS and SW1353 cells were then assessed by Cell Counting Kit-8 and transwell assay, respectively. The interaction between circCCDC66 and its target miRNAs were verified by the dual-luciferase reporter assay. Through functional experiments, we found that circCCDC66 knockdown promoted the inhibition of cell proliferation and metastatic of OS cell lines. From mechanistic perspective, circCCDC66 upregulated PTP1B by sponging miR-338-3p. Collectively, our findings demonstrated that circCCDC66 contributed to malignant behaviors of OS cells by miR-338-3p/PTP1B pathway, which suggested circCCDC66/miR-338-3p/PTP1B axis might be a potential therapeutic target.
Highlights
In addition to the mRNA involved in coding, there are a large number of noncoding RNAs
Studies have demonstrated the potential value of Aurora A kinase as a tumor marker due to its high expression in OS and the potential of plasma 4 integrin and ezrin as tumor markers in the initial stage of OS development [21,22,23]
With the rapid development of biology, more and more RNA biomolecules are used as clinical markers of cancer [24]
Summary
In addition to the mRNA involved in coding, there are a large number of noncoding RNAs. As for the origin of circRNA, with the deepening of research, we gradually realize the particularity and complexity of its biogenesis mechanism. Compared with previous studies, researchers have made some progress in the study of circRNA functions [1, 5]. CircRNA can combine the competition of miRNA with the downstream target genes to achieve the inhibitory effect of miRNA, modulating the downstream target genes expression [6]. Intron circRNAs can affect parental genes transcription in the nucleus. The exon circRNA itself is a product of the regulatory mechanism, and it is spliced into a ring, so the expression of its parent genes must be affected [5]. CircRNA must have other features that we have not yet learned in detail
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