Abstract
BackgroundCircular RNA (circRNA) CDR1as plays an important role in the occurrence and development of human tumors. The purpose of this study is to investigate the molecular mechanism of circRNA CDR1as in the development of nasopharyngeal carcinoma (NPC).MethodsThe mRNA expressions of circRNA CDR1as, miR-7-5p, and E2F3 were detected by qRT-PCR. The effects of circRNA CDR1as, miR-7-5p, and E2F3 on NPC cells were investigated using cell counting kit-8 (CCK8) method, colony formation assay, and representative metabolite assay. The molecular mechanism of circRNA CDR1 in NPC was studied by bioinformatics and luciferase reporter assay. In addition, the biological activity of circRNA CDR1as was also investigated in NPC xenograft tumor mice model.ResultsThe results showed that the circRNA CDR1as expression was significantly up-regulated in NPC tissues by comparison with non-tumor NPE tissues (p < 0.01), suggesting that circRNA CDR1as was associated with poor prognosis in NPC patients. Moreover, circRNA CDR1as could up-regulate E2F3 expression by binding miR-7-5p, and promote the growth and glucose metabolism of NPC cells. Meanwhile, circRNA CDR1as could promote NPC progression through the negative regulation of miR-7-5p in the xenograft tumor model.ConclusionCircRNA CDR1as promoted the occurrence and development of NPCs by successively up-regulating the expression of miR-7-5p and E2F3, suggesting CircRNA CDR1as as a potential target for the treatment of NPC patients.Trial registration The study was approved by the cancer center’s institutional research ethics committee on Oct 18, 2008 (2008GZ2847462)
Highlights
Circular RNA Cerebellar degeneration associated protein 1 antisense transcript (CDR1as) plays an important role in the occurrence and development of human tumors
Pearson correlation analysis showed that the high expression of circular RNA (circRNA) CDR1as in the tissues of nasopharyngeal carcinoma (NPC) patients was correlated with clinical staging (p < 0.01), but not related to other factors (Table 1)
The above results confirmed that circRNA CDR1as was highly expressed in NPC tissues and cell lines, which might be related to the promotion of NPC development
Summary
Circular RNA (circRNA) CDR1as plays an important role in the occurrence and development of human tumors. The purpose of this study is to investigate the molecular mechanism of circRNA CDR1as in the development of nasopharyngeal carcinoma (NPC). As competitive endogenous RNAs (ceRNA), CircRNAs can bind to miRNAs and inhibit their functions [11]. Recent studies have found that miR-7-5p is an endogenous tumor suppressor gene that can target and inhibit lymphoma, gastric cancer, glioma, and other malignant tumors [14, 15]. CircRNA CDR1as contains 70 binding sites of miR-7 and can inhibit the function of miR-7 and indirectly promote the stability of miR-7 target genes [16,17,18,19]
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