Abstract
Avian leukosis virus subgroup J (ALV-J) can induce myeloid tumors and hemangiomas in chickens and causes severe economic losses with commercial layer chickens and meat-type chickens. Here, we generated ribominus RNA sequencing data from three normal chicken spleen tissues and three ALV-J-infected chicken spleen tissues. Structure analysis of transcripts showed that, compared to mRNAs and lncRNAs, chicken circRNAs shared relatively shorter transcripts and similar GC content. Differentially expression analysis showed 152 differentially expressed circRNAs with 106 circRNAs up regulated and 46 circRNAs down regulated. Through comparing differentially expressed circRNA host genes and mRNAs and performed ceRNA network analysis, we found several tumor or immune-related genes, in which, there were four genes existed in both differentially expressed mRNAs and circRNA host genes (Dock4, Fmr1, Zfhx3, Ralb) and two genes (Mll, Aoc3) involved in ceRNA network. We further characterized one exon-intron circRNA derived from HRH4 gene in the ceRNA network, termed circHRH4, which is an abundant and stable circRNA expressed in various tissues and cells in chicken and localizes in cytoplasm. Our results provide new insight into the pathology of ALV-J infection and circRNAs may also mediate tumorigenesis in chicken.
Highlights
Avian leukosis virus (ALV) is an avian oncogenic retrovirus, which belongs to genus Alpharetrovirus, subfamily Orthoretrovirinae within the Retroviridae family, leads to neoplastic diseases and other reproduction problems in the poultry industry worldwide
Though Avian leukosis virus subgroup J (ALV-J) has been eradicated from breeding flocks successfully in the western world, it has become more pervasive throughout China in recent decades [6]
CircRNAs and competing endogenous RNAs (ceRNAs) networks involved in ALV-J-induced tumorigenesis, in this study, spleen tissues of six 20-week-old female bone silky fowls (BSFs) with (n = 3) and without (n = 3) spontaneous ALV-J infection were subjected to RNA sequencing (RNA-Seq) and small RNA-Seq analyses
Summary
Avian leukosis virus (ALV) is an avian oncogenic retrovirus, which belongs to genus Alpharetrovirus, subfamily Orthoretrovirinae within the Retroviridae family, leads to neoplastic diseases and other reproduction problems in the poultry industry worldwide. It can be classified as endogenous virus (subgroup E) or exogenous virus (subgroup A, B, C, D, and J) according to viral envelope interference, host range, cross-neutralization patterns and mode of transmission[1]. ALV-J infected hosts are characterized as delayed. Circular RNA and mRNA profiling of ALV-J-infected chickens
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