Abstract
Non-small cell lung cancer (NSCLC) is a common type of lung cancer, characterized by a poor prognosis. In the last several years, more and more studies have demonstrated the significant roles played by circular RNAs (circRNAs) in different human tumors progression including NSCLC. The present study was to explore the mechanism of hsa_circ_101237 in regulating non-small cell lung cancer (NSCLC). Totally 303 NSCLC cases were enrolled. A549 and H1299 cells were transfected. Cells viability, migration and invasion were determined by CCK-8 assay and transwell experiment, respectively. Luciferase reporter gene assay and RNA immunoprecipitation (RIP) assay were performed. hsa_circ_101237, miR-490-3p and MAPK1 expression in tissues/cells were detected by qRT-PCR. The study found an elevation in the expression of Hsa_circRNA_101237 in both NSCLC tissues and cell line. High Hsa_circRNA_101237 expression predicted poor survival in NSCLC. Meanwhile, we found that hsa_circRNA_101237 expression sponged miR-490-3p to enhance MAPK1 expression, thus significantly promoting NSCLC cell lines proliferation, migration, and invasion. MAPK1 restoration prevented NSCLC cells proliferation, migration, and invasion to be repressed due to hsa_circRNA_101237 knockdown. To sum up, as revealed by the study, hsa_circRNA_101237 promoted the expression of MAPK1 via miRNA-490-3p sponge, thus affecting the NSCLC as an important onco-circRNA.
Highlights
Lung cancer, characterized by a poor prognosis but a high morbidity, is a common malignant tumor, non-small cell lung cancer (NSCLC) are identified as the most common type of lung cancer[1]
Many human cancers see altered circRNA expression, and many researches have revealed the key role played by circRNAs in the tumorigenesis. circRNA_101237, a new circRNA identified recently, whose encoding gene is located at chromosome 13:26974589-26975761 and which is produced by backsplicing of exons 10, 11 and 12 of cyclin-dependent kinase (CDK) 8, has been reported to be implicated in Cisplatin resistance-associated of HCC8
QRT-PCR assay was conducted aiming at characterizing the hsa_circ_101237 in NSCLC tissues, which demonstrated that NSCLC tissues held an obviously higher hsa_circ_101237 expression compared with nearby non-tumor tissues (Fig. 1A,B)
Summary
Lung cancer, characterized by a poor prognosis but a high morbidity, is a common malignant tumor, non-small cell lung cancer (NSCLC) are identified as the most common type of lung cancer (occupying about 85%)[1]. More and more clinical studies found that metastasis greatly hinders the treatment of NSCLC cancer; in-depth understanding the metastasis mechanisms is beneficial for effectively treating NSCLC. CircRNAs have a special closed loop structure, making them able to resist the degradation medicated by exonuclease. On that account, they are more stable compared with most other linear RNAs, they can be used as a biomarker to effectively diagnose and treat cancers. The study focused on illuminating how hsa_circRNA_101237 affects the pathogenesis of NSCLC, as well as the regulating mechanism in vitro. In terms of the function, hsa_circRNA_101237 facilitated the development, the migration and the invasion of NSCLC cell. In terms of the mechanism, hsa_circRNA_101237 regulated the miR-490-3p/MAPK1 axis, and contributed to NSCLC progression. Clinical features Age (years)
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