Abstract
BackgroundDysregulation of circRNAs has been reported to be functionally associated with chronic obstructive pulmonary disease (COPD). The present investigation elucidated the potential role of CircRNA0001859 in regulating chronic obstructive pulmonary disease acute (COPD) and Acute Exacerbation of COPD (AECOPD).MethodsMice model of COPD was established to screen and verify the dysregulated expression of CircRNA0001859. Fluorescence in situ hybridization (FISH) and quantitative real-time PCR (qRT-PCR) were carried out to detect the expression of CircRNA0001859. 38 stable COPD patients, 24 AECOPD patients, 57 COPD with lung cancer patients and 28 healthy person with age and sex matched to total patients were used for the present investigation.ResultscircRNA0001859 was downregulated in the lung tissue of mice after the three kinds of treatments (Cigarette smoke (CS)/NK alone or CS + NNK) for inducing COPD. FISH assay verified the downregulation of circRNA0001859 both in the mice lung and human bronchial epithelial cell of COPD model. Furthermore,, the level of circRNA0001859 was also downregulated in the peripheral blood of COPD and lung cancer patients. CircRNA0001859 might act as a diagnostic and prognostic biomarker for the treatment of in COPD and AECOPD with Are under the receiver operating characteristic curve (ROC curve) (AUC) of 0.7433 and 0.8717, respectively.ConclusionWe explored a novel circRNA0001859, which might act as a potential therapeutic biomarker for the treatment of COPD and AECOPD.
Highlights
Dysregulation of circRNAs has been reported to be functionally associated with chronic obstructive pulmonary disease (COPD)
The values of Forced Expiratory Volume in 1 second (FEV1) (L), FEV1/Forced Vital Capacity (FVC) (%) and FEV1% predicted in Chronic obstructive pulmonary disease (COPD), Acute Exacerbation of COPD (AECOPD) and COPD patients with lung cancers were remarkably reduced as compared to the Healthy controls (HCs) group (P < .001) (Table 1)
CircRNA0001859 was down-regulated in lung tissue of COPD mouse To investigate the role of circRNA in the progression of COPD, we established the mouse model of COPD using cigarette smoke (CS) treatment lasting for 14 weeks
Summary
Dysregulation of circRNAs has been reported to be functionally associated with chronic obstructive pulmonary disease (COPD). CircRNAs for the first time are observed in the early 1970s in plant viroid and they were considered as transcriptional “noise” with no biological function but the recent emerging evidences have shown that it plays a crucial role in physiological and pathological processes and due to their abundance and stability [7,8,9]. They have been considered as potential prognostic and diagnostic biomarkers in diverse range of diseases [10]. CircRNAs regulate these biological processes by sponging miRNAs, protein coding, alternative splicing, transcriptional and posttranscriptional regulations inhibit cell cycle protein bait function and circRNA derived pseudogenes [11, 12]
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