Abstract
Objective: Circular RNAs (circRNAs) have been demonstrated in playing an important role in the physiological and pathological processes (such as cancer). This paper aims to clarify the role of Circ_0006677 in non–small-cell lung cancer (NSCLC) progression. Methods: Using clinical data and in vitro cell line models, we revealed the tumor-suppressive role of circ_0006677 in lung cancer. Using the online bioinformatics tool, we predicted the target of circ_0006677 and further validated its regulatory mechanisms responsible for its tumor suppressor function in NSCLC. Results: Circ_0006677 expression was reduced in NSCLC tissues of patients and lung cancer cells in comparison to adjacent normal tissues. Lower expression of circ_0006677 was significantly associated with poorer patient survival. Overexpression of circ_0006677 significantly inhibited the ability of NSCLC cell proliferation, migration, invasion, and glycolysis. Mechanically, circ_0006677 could inhibit NSCLC progression and glycolysis by regulating the expression of the signal transducer inhibitor SOSC2 through sponging microRNA-578 (miR-578). Conclusion: Circ_0006677 prevents the progression of NSCLC via modulating the miR-578/SOSC2 axis.
Highlights
Lung cancer is the leading cause of cancer-related deaths worldwide (Sève et al, 2010)
We found that circ_0006677 was downregulated in Non-smallcell lung cancer (NSCLC) tissues compared with adjacent normal tissues (Figure 1A)
The qRT-PCR assays revealed that the expression of circ_0006677 was significantly lower in a series of NSCLC cell lines than the HBE (Figure 1D)
Summary
Lung cancer is the leading cause of cancer-related deaths worldwide (Sève et al, 2010). Circ_0006677 Inhibits NSCLC Progression et al, 2010). CircRNAs were shown to regulate multiple biological processes by functioning as microRNA (miRNA) sponges (Hansen et al, 2013; Wan et al, 2016), interacting with RNA-binding proteins (Du et al, 2016), mediating gene transcription (Li et al, 2015) and protein translation (Abe et al, 2015; Mentha et al, 2019). Abnormal expression of circRNAs has been reported in many cancers and is involved in the regulation of cancer progression (Meng et al, 2017; Bach et al, 2019). In many types of cancer, miR-578 was reported to promote cancer development (Chen et al, 2020; Ji et al, 2020; Wu et al, 2020). SOCS2 is a member of the suppressor of cytokine signaling (SOCS) family and represses the cytokine-induced signaling transduction, inhibiting cancer progression (Ricobautista et al, 2006; Das et al, 2017; Chhabra et al, 2018; Tong et al, 2019)
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