CircRNA ACVR2A Sponges miR-1290 to Modulate Cell Progression in Gastric Cancer.

YingYing Zhuang,HaiNing Wu,TaiYong Fang,LiYa Li,Manu Kanjoormana Aryan

doi:10.1155/2022/9461054

YingYing Zhuang, HaiNing Wu + Show 3 more

Open Access

https://doi.org/10.1155/2022/9461054

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Abstract

Background In recent years, the abnormal expression of circRNAs has been identified to be strongly associated with tumor tissues. In this study, we focused on circACVR2A with a remarkably upregulated expression in gastric tissues and further explored its role in the pathogenic progression of gastric cancer (GC). Methods The differentially expressed circACVR2A in GC tissues and four cell lines (MKN-45, SNU-1, HGC-27, and SGC-7901) was identified by qRT-PCR method. Then, the effect of circACVR2A and miR-1290 on HGC-27 cell proliferation was measured by CCK8 and the colony formation methods. The effect of circACVR2A and miR-1290 on HGC-27 cell metastasis was estimated by transwell assay. The interaction of circACVR2A and miR-1290 was further detected. Results The relative level of circACVR2A in GC tissues and cell lines is remarkably upregulated. The downregulation of circACVR2A promotes GC cell proliferation and metastasis and suppressed the expression level of E-cadherin and Vimentin. The miR-1290 inhibitor reversed the effect of circACVR2A on cell progression in GC cell. Conclusion circACVR2A competitively sponged miR-1290 and was exerted as a tumor suppressor gene oncogene via a circACVR2A/miR-1290 axis, suggesting it as a possible biomarker for GC therapy.

Highlights

Gastric cancer is the most common type of diagnosed cancer around the world, with a high mortality rate, accounting for 8.2% of all cancer deaths [1]
We revealed that circACVR2A was upregulated in gastric cancer (GC) tissues and four cell lines. e circACVR2A could influence GC cell proliferation and metastasis via interacting with miR-1290 as a miRNA sponge
Aberrant Upregulation of circACVR2A in GC Tissues and Cell Lines. e relative level of circACVR2A in GC tissues (n 20) and cell lines was estimated. circACVR2A exhibited a significant upregulation in contrast to the adjacent tissues (n 20; p < 0.01; Figure 1(a)). e similar findings have been observed from the GC cell lines (MKN-45, SNU-1, SGC7901, and HGC-27) in contrast to the normal gastric epithelial cell (GES-1; Figure 1(b))

Summary

Introduction

Gastric cancer is the most common type of diagnosed cancer around the world, with a high mortality rate, accounting for 8.2% of all cancer deaths [1]. We focused on circACVR2A with a remarkably upregulated expression in gastric tissues and further explored its role in the pathogenic progression of gastric cancer (GC). E differentially expressed circACVR2A in GC tissues and four cell lines (MKN-45, SNU-1, HGC-27, and SGC-7901) was identified by qRT-PCR method. En, the effect of circACVR2A and miR-1290 on HGC-27 cell proliferation was measured by CCK8 and the colony formation methods. E effect of circACVR2A and miR-1290 on HGC-27 cell metastasis was estimated by transwell assay. E relative level of circACVR2A in GC tissues and cell lines is remarkably upregulated. E downregulation of circACVR2A promotes GC cell proliferation and metastasis and suppressed the expression level of E-cadherin and Vimentin. Conclusion. circACVR2A competitively sponged miR-1290 and was exerted as a tumor suppressor gene oncogene via a circACVR2A/miR-1290 axis, suggesting it as a possible biomarker for GC therapy

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Conclusion