Abstract
BackgroundCircular RNA nuclear factor I X (circNFIX) has been reported to play an important role in glioma progression. However, the mechanism by which circNFIX participates in glioma progression remains poorly understood.MethodsGERIA online were used to analyze the abnormally expressed genes in glioma tissues. The expression levels of circNFIX, microRNA (miR)-378e and Ribophorin-II (RPN2) were measured by quantitative real-time polymerase chain reaction or western blot. Cell cycle distribution, apoptosis, glycolysis, migration and invasion were determined by flow cytometry, special kit and trans-well assays, respectively. The target association between miR-378e and circNFIX or RPN2 was confirmed by luciferase reporter assay, RNA immunoprecipitation and pull-down. Xenograft model was established to investigate the role of circNFIX in vivo.ResultsThe expression of circNFIX was enhanced in glioma tissues and cells compared with matched controls and high expression of circNFIX indicated poor outcomes of patients. Knockdown of circNFIX led to arrest of cell cycle, inhibition of glycolysis, migration and invasion and promotion of apoptosis in glioma cells. circNFIX was a sponge of miR-378e. miR-378e overexpression suppressed cell cycle process, glycolysis, migration and invasion but promoted apoptosis. miR-378e silence abated the suppressive role of circNFIX knockdown in glioma progression. RPN2 as a target of miR-378e was positively regulated via circNFIX by competitively sponging miR-378e. Silencing circNFIX decreased glioma xenograft tumor growth by regulating miR-378e/RPN2 axis.ConclusionKnockdown of circNFIX inhibits progression of glioma in vitro and in vivo by increasing miR-378e and decreasing RPN2, providing a novel mechanism for understanding the pathogenesis of glioma.
Highlights
Circular RNA nuclear factor I X has been reported to play an important role in glioma progression
Results circNFIX expression is increased and indicates poor outcomes of patients in glioma The data of heat map displayed 23 differentially expressed circRNAs including down-regulated and upregulated circRNAs, of which circNFIX was significantly increased in glioma tissues (Fig. 1a)
Compared with that in human astrocyte cell line (HA) cells, the abundance of circNFIX was notably up-regulated in glioma cells, especially in T98 and U251 cells (Fig. 1c)
Summary
Circular RNA nuclear factor I X (circNFIX) has been reported to play an important role in glioma progression. The mechanism by which circNFIX participates in glioma progression remains poorly understood. Despite many advances in the treatment of glioma, the effective strategies remain limited. CircRNAs are a member of noncoding RNAs playing essential roles in various cancers through regulating multiple biological processes, including proliferation, apoptosis, cell cycle process, migration and invasion [2], Ding et al Journal of Experimental & Clinical Cancer Research (2019) 38:506 of multiple organ systems, including brain [10, 11]. The corresponding circRNA NFIX (circNFIX) could promote cell proliferation in glioma by regulating miR-34a-5p [12]. The influence and mechanism of circNFIX in glioma need more researches
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Experimental & Clinical Cancer Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
