Abstract
Circular RNAs (circRNAs) are a panel of non-coding RNAs that mediate the regulation of gene expression, as well as pathological responses. Nonetheless, the function and expression pattern of circRNAs in urinary bladder cancer (UBC) remain unclear. Herein, we examined the function of circCA12 in UBC development. qRT-PCR results demonstrated remarkable circCA12 upregulation in UBC cell lines, as well as tissues. CCK-8, colony formation, and xenograft assays were employed to determine the effect of circCA12 on UBC. Our data illustrated silencing circCA12 repressed the proliferation along with the colony-formation capability of UBC cells. The migration and metastasis potential of UBC cells were remarkably abated in vivo, as well as in vitro after transfection with si-cirCA12 or sh-circCA12. Moreover, luciferase reporter and RIP assays indicated that circCA12 binds to miRNA-1184 through sponging miRNA, thereby up-regulating the expression of RAS family genes (NRAS, KRAS, and HRAS). In conclusion, the circCA12/miRNA-1184/RAS family was identified as a regulatory axis in UBC progression.
Highlights
Urinary bladder cancer (UBC) is responsible for about 549,000 new cases, as well as 200,000 deaths every year
We explored circCA12 expression level using four bladder cell lines
We found that down-regulation of circCA12 significantly repressed the growth, migration, and infiltration of UBC cells
Summary
Urinary bladder cancer (UBC) is responsible for about 549,000 new cases, as well as 200,000 deaths every year. It is the sixth most prevalent cancer in men and is ranked ninth among the main causes of cancer-linked deaths (Bray et al, 2018). Reports indicate that it is the most frequent malignant tumor in the urinary system (Antoni et al, 2017).
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