Abstract
This study was performed to research circB3GNTL1 control, miR-598 and its mechanism for cell proliferation, apoptosis and glutamine breakdown. For this purpose, CirB3GNTL1 and miR-598 expressions were detected by qRT-PCR in gastric and cell lines; MTT tests were performed to detect proliferation; flow cytometry was established in flow; glutamine decomposition was evaluated with glutamine, glutamic acid and α-keto-glutaric acid (α-KG) expression; Bcl-2, PCNA, ASCT2 and GLS1 expression levels were calculated; Methods of expression were calculated. The results showed that CircB3GNTL1 expression was up-regulated and miR-598 expression was up-regulated in gastric cancer tissues and cell lines; Knockdown circB3GNTL1 prevented proliferation and glutamine decomposition of the gastric cancer cells and induced apoptosis compared with normal para-cancers and gastric cancer cell lines. Results circB3GNTL1 can target and control miR-598 expression, and miR-598 can reverse the proliferation, apoptosis, and decomposition of glutamine from gastric cancer cells by knockdown circB3GNTL1. It was concluded that CirB3GNTL1 prevents decomposition of glutamines and induces apoptosis by controlling miR-598 in gastric cancer cells.
Highlights
Gastric cancer has high morbidity, high mortality, and poor prognosis and survival [1, 2] as one of the common malignancies tumours of the digestive system
CirB3GNTL1 and miR-598 expressions were detected by qRT-PCR in gastric and cell lines; MTT tests were performed to detect proliferation; flow cytometry was established in flow; glutamine decomposition was evaluated with glutamine, glutamic acid and α-keto-glutaric acid (α-KG) expression; Bcl2, PCNA, ASCT2 and GLS1 expression levels were calculated; Methods of expression were calculated
The results showed that CircB3GNTL1 expression was up-regulated and miR-598 expression was up-regulated in gastric cancer tissues and cell lines; Knockdown circB3GNTL1 prevented proliferation and glutamine decomposition of the gastric cancer cells and induced apoptosis compared with normal para-cancers and gastric cancer cell lines
Summary
Gastric cancer has high morbidity, high mortality, and poor prognosis and survival [1, 2] as one of the common malignancies tumours of the digestive system. CircumRNA expression is closely linked to the incidence and progression of gastric cancer in gastric cancer cells [10]. The silence of circum-DONSON expression can inhibit gastric cancer cell growth and metastasis. Zhang et al.’s research results [12] have found that circDLST can function in gastric cancer cells as the sponge of miR-502-5p, and influence the spread and metastasis of gastric cancer cells. A new concept for cancer diagnosis and treatment can be given to MiRNA's proliferation and apoptosis of cancer cells [13]. This study, found that circB3GNTL1 influences the propagation, apoptosis and glutamine decomposition of gastric cells by modulation of the miR-598 expression, in order to achieve a new goals for clinical diagnosis and treatment of gastric cancer. CirB3GNTL1 was the potential mechanical mechanism for gastric cancer cells
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