Abstract
Background: CircRNAs have been found to be involved in the pathogenesis of various diseases. We aimed to explore the roles of circRNAs in ovarian cancer. Methods: The expression levels of circRNAs in ovarian cancer and normal ovarian tissues were analyzed using RNA sequencing. Fluorescent in situ hybridization (FISH), proliferation assays and transwell assays were used to assess the effects of circRNAs on ovarian cancer. Results: CircATRNL1 and circZNF608 were downregulated in 20 ovarian cancer tissues compared to normal tissues. CircATRNL1 and circZNF608 are mainly located in the cytoplasm of ovarian cancer cells, and circATRNL1 is a highly conserved circRNA. The overexpression of circATRNL1 and circZNF608 inhibits the proliferation and invasion of ovarian cancer cells. We predicted miRNA–circRNA interactions for circZNF608 and circATRNL1 and obtained 63 interactions. However, a luciferase reporter assay showed that only miR-152-5p was sequestered by circZNF608. Bioinformatics analysis and experiments indicated that circATRNL1 contains an internal ribosome entry site and an open reading frame encoding a 131 aa protein. Conclusion: In conclusion, circATRNL1 and circZNF608 are two downregulated circRNAs in ovarian cancer and work as tumor suppressors. CircZNF608 may exert antitumor activity in ovarian cancer by binding miR-152-5p, and circATRNL1 may encode a 131 aa protein.
Highlights
Ovarian cancer is the seventh most prevalent female cancer worldwide (Vafadar et al, 2020)
We explored the effects of circZNF608 and circATRNL1 on ovarian cancer cells
We further explored circATRNL1 and circZNF608 on invasion and found that the overexpression of circATRNL1 and circZNF608 inhibited the invasion of ovarian cancer cell lines (Figures 2F,G)
Summary
Ovarian cancer is the seventh most prevalent female cancer worldwide (Vafadar et al, 2020). Ovarian cancer is usually associated with silent and unclear symptoms, which makes it a difficult disease to diagnose, and it is usually diagnosed in advanced stages (Jayson et al, 2014). Standard treatment options for ovarian cancer include surgery and chemotherapy (Stewart et al, 2019). Current treatments are unable to cure advanced ovarian cancer. Early diagnosis represents the best hope for mortality reduction and long-term disease control (Rauh-Hain et al, 2011). The. CircRNAs have been found to be involved in the pathogenesis of various diseases. We aimed to explore the roles of circRNAs in ovarian cancer
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